Genomics & Genetics Weekly
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Fusion Proteins
Investigators at Nanjing Normal University publish new data on fusion proteins
July 4th, 2008
According to recent research from Nanjing, People's Republic of China, "Elevated levels of B-cell-activating factor of the TNF family (BAFF) have been implicated in the pathogenesis of autoimmune diseases in human. In this study, an anti-BAFF single-chain antibody fragment (scFv) was genetically linked to the C terminus of the enhanced green fluorescent protein (EGFP) to generate an EGFP/scFv fusion protein." "The EGFP/scFv fusion protein had an apparent molecular weight of 52 kDa and was identified by sodium dodecyl sulfate polyacrylamide gel electrophoresis and Western blot analysis. After being purified by immobilized metal affinity chromatography, the fusion protein...
Source: Genomics & Genetics Weekly (2008-07-04)
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