Genomics & Genetics Weekly
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Escherichia coli
Research conducted at H. Yu and co-authors has provided new information about Escherichia coli
December 12th, 2008
According to recent research from the United States, "Hyaluronic acid (HA) is an important biomaterial with functional medical and cosmetic applications. As its synthesis has been recently reported in recombinant bacteria, it is of interest to develop a high throughput screening method for the rapid isolation of HA accumulating strains transformed by combinatorial libraries." "Here we report a novel two-step screening strategy to select for better HA-producing recombinant Escherichia coli strains transformed by mutation libraries of rpoD and rpoS, coding for the sigma(D) and sigma(s) factors of the RNA polymerase, respectively. The first screen, based on translucent...
Source: Genomics & Genetics Weekly (2008-12-12)
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