Columbia University
Yeast three-hybrid assays suitable for use in drug discovery
Published in Health Business Week, October 15th, 2004
"The yeast two-hybrid assay has proven to be a powerful method to detect protein-protein interactions as well as to derive genome-wide protein interaction maps," scientists in New York explained. "More recently, three-hybrid assays have emerged as a means to detect both protein-RNA and protein-small molecule interactions."
"Despite the routine use of the two-hybrid assay and the potential of three-hybrid systems, there has been little quantitative characterization to understand how the strength of the protein interaction correlates with transcription activation," noted K.S. de Felipe...
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Source: Health Business Week (2004-10-15)
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