Institute Gustave Roussy
A real-time PCR assay screens for p53 recombinant adenovirus in patient samples
Published in Health Risk Factor Week, January 12th, 2004
"The purpose of this study was to assess the usefulness of real-time polymerase chain reaction PCR as a quantitative, highly reproducible, and sensitive method for detecting and quantifying p53 recombinant adenovirus in biological samples from cancer patients receiving injections of adenovirus type-5 - cytomegalovirus (Ad5CMV)-p53. The dynamic range of this real-time PCR-based assay was wide (at least five orders of magnitude). Our assay used an internal positive control in the same PCR tube that is capable of detecting residual PCR inhibitors," according to researchers in France.
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