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Baylor University

Assay for melanoma-specific CTL from dendritic cell vaccines developed

Published in Health Risk Factor Week, May 10th, 2005

Researchers have developed a method for measuring melanoma-specific cytotoxic T lymphocytes (CTL) elicited by dendritic cell vaccines.

"Improving cancer vaccines depends on assays measuring elicited tumor-specific T-cell immunity. Cytotoxic effector cells are essential for tumor clearance and are commonly evaluated using 51Cr release from labeled target cells after a short (4 hours) incubation with T cells. The authors used a tumor inhibition assay (TIA) that assesses the capacity of cytotoxic T lymphocytes (CTLs) to control the survival/growth of EGFP-labeled tumor cell lines. TIA was validated using CD8+ T cells primed in vitro against melanoma and breast...

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