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Antisense Technology
New MAST technology has universal applications for tracking desired mRNA sites
September 1st, 2003
New MAST technology has universal applications for tracking desired mRNA sites. "A solution-based method, mRNA accessible site tagging (MAST), has been developed to map the accessible sites of any given mRNA in high throughput fashion. mRNA molecules were immobilized and hybridized to randomized oligonucleotide libraries. Oligonucleotides specifically hybridized to the mRNA were sequenced and found to be able to precisely define the accessible sites of the mRNA," scientists in Sweden report. "A number of ways were used to validate the accessible sites defined by the MAST process. Mapping of rabbit beta-globin mRNA demonstrates the efficacy and...
Source: Health & Medicine Week (2003-09-01)
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