Airway Inflammation

Induced sputum can be used in PCR to assay for cytokines in airway inflammation

Published in Health and Medicine Week, December 1st, 2003

Induced sputum having few cells can be used to assay for cytokines by PCR, to monitor airway inflammation noninvasively.

According to recent research from France, "sputum examination is being increasingly used as a noninvasive method for the study of airway inflammation. However, the technical applications of sputum are still limited because of the small number of cells recovered.

"In an attempt to extend applications of sputum examinations, we developed and standardized an RT-PCR, a sensitive and specific technique of detection of mRNA, in induced sputum samples. Total RNA could be extracted from samples containing as few as 50-80 x 10³...

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Source: Health and Medicine Week (2003-12-01)

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