Published in Health and Medicine Week, December 1st, 2003
According to recent research from France, "sputum examination is being increasingly used as a noninvasive method for the study of airway inflammation. However, the technical applications of sputum are still limited because of the small number of cells recovered.
"In an attempt to extend applications of sputum examinations, we developed and standardized an RT-PCR, a sensitive and specific technique of detection of mRNA, in induced sputum samples. Total RNA could be extracted from samples containing as few as 50-80 x 103...
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