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Health & Medicine Week

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Biotechnology



DNA chip offers simultaneous detection of HIV, HBV and HCV in blood



March 22nd, 2004

A novel DNA chip enables simultaneous detection of HIV, HBV, and HCV in blood samples.

According to recent research from China, "For the simultaneously visual detection of hepatitis B virus (HBV), hepatitis C virus (HCV) and human immunodeficiency virus type-1 (HIV-1), a qualitative DNA chip method, combining multiplex and nested polymerase chain reaction (PCR) with arrayed anchored primer PCR and a biotin-avidin alkaline phosphatase (Av-AP) indicator system, was developed."

"After pretreatment of infected blood samples and reverse transcription of the RNA virus genome, PCR was performed in a single tube by using the outer primer pairs," explained...


Source: Health & Medicine Week (2004-03-22)

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