Murine Leukemia Virus
Real-time PCR evaluates X-MuLV removal during protein purification
Published in Health and Medicine Week, November 22nd, 2004
According to published research from the United States, "Chinese hamster ovary cells used for pharmaceutical protein production express noninfectious retrovirus-like particles. To assure the safety of pharmaceutical proteins, validation of the ability of manufacturing processes to clear retrovirus-like particles is required for product registration."
"Xenotropic murine leukemia virus (X-MuLV) is often used as a model virus for clearance studies. Traditionally," wrote L.M. Shi and coworkers, "cell-based infectivity assay has been the standard virus quantification method. In this...
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