Accutane
Isotretinoin levels after single oral dose determined by HPLC
Published in Health and Medicine Week, May 2nd, 2005
According to recent research from the United States, "An isocratic reverse-phase high-performance liquid chromatographic method was established and validated according to FDA's Guidance for Industry, 'Bioanalytical Method Validation,' for the determination of isotretinoin in plasma and brain tissue from mice following single and multiple oral doses of Accutane. Plasma sample preparation included deproteination with acetonitrile-perchloric acid followed by centrifugation. Brain tissue was homogenized and extracted with...
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