Stem Cell Research
Stem cell factor fused with mimetic peptide in Escherichia coli
Published in Health and Medicine Week, August 14th, 2006
"TPO acts synergistically with SCF in hematopoiesis and megakaryopoiesis. In this work, we designed the expression of SCF fused with the monomer or the dimer of TPO mimetic peptide through a flexible peptide linker.
"The recombinant fusion proteins were produced in E. coli DH5 alpha at up to 25% of total cell proteins. The resultant inclusion bodies were refolded by dilution and purified by ion-exchange chromatography," wrote L. Su and colleagues,...
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