University of Rome la Sapienza
One-step nested PCR monitors HIV replication
Published in Healthcare Finance, Tax and Law Weekly, June 15th, 2005
According to recent research published in the Journal of Virological Methods, "A highly sensitive nested PCR was carried out in order to detect 2 LTR circles as a marker of recent and ongoing vital replication in HIV-1 infected patients with HIV plasma RNA undetectable.
"This 'in house' 2-step nested PCR is very sensitive, but it is not feasible for routine tests and presents a high risk of contamination."
"In order to reduce the time of reactions and crossover contamination, the possibility was explored to carry out a single step nested PCR, in which the...
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