Published in Hematology Week, July 30th, 2007
"We hypothesized that the normally discarded BM collection kits might be a convenient source of large numbers of MSC. Marrow specimens were isolated from used Fenwal collection kits. Purified mononuclear cells (MNC) were screened by multiparameter flow cytometry to identify MSC, which were later expanded by in vitro culture. Immunophenotyping and differentiation assays were performed initially and at subculture. Both fresh and frozen BM were...
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