"DNA Single Strand Conformation Polymorphism Identifies Five Defined Strains of Hepatitis B Virus (HBV) during an Outbreak of HBV Infection in an Oncology Unit."

According to the authors' abstract of an article published in Journal of Medical Virology, "An outbreak of hepatitis B virus (HBV) infection in a children's oncology unit was identified in which 61 children were shown to have been infected, 59 of them asymptomatically. In order to establish whether intra-unit cross infection had occurred, we used the single strand conformational polymorphism (SSCP) technique to analyze viral isolates from 57 of the infected chiIdren and 40 unrelated controls. HBV-specific primers were designed to amplify a 189 bp fragment of DNA encompassing part of the hypervariable pre-S1 region of the HBV genome. Denatured PCR products were compared after...

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