Food-Borne Illness
"A Virion Concentration Method for Detection of Human Enteric Viruses in Oysters by PCR and Oligoprobe Hybridization."
Published in Hepatitis Weekly, July 15th, 1996
According to the authors' abstract of an article published in Applied and Environmental Microbiology, "This article reports the development of a method to purify and concentrate intact virions from oyster extracts to a volume and quality compatible with viral genomic nucleic acid amplification by reverse transcriptase PCR (RT-PCR) and confirmation by oligonucleotide probe hybridization. Fifty-gram oyster samples were processed by an adsorption-elution-precipitation method and then seeded with 101 to 105 PFU of poliovirus type 1 (PV1) or hepatitis A virus (HAV). Seeded viruses in oyster extracts were purified by fluorocarbon extraction and concentrated by...
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Source: Hepatitis Weekly (1996-07-15)
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