Diagnostics (HCV)

Three Procedures Reliable in Genotyping Hepatitis C

Published in Hepatitis Weekly, October 28th, 1996

Type-specific polymerase chain reaction testing, molecular hybridization and enzyme-linked immunosorbent assay (ELISA) for type-specific antibodies are all reliable procedures for investigating hepatitis C virus genotypes, according to a report from Spain.

Several methods have been developed to identify hepatitis C virus (HCV) genotypes, including PCR amplification of HCV cDNA with type-specific primers, restriction fragment length polymorphism analysis of PCR amplicons, molecular hybridization of type-specific probes to PCR-amplified HCV cDNA, genome amplification and sequencing and identification of type-specific antibodies using ELISA.

"Since all...

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Source: Hepatitis Weekly (1996-10-28)

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