Genotyping (HCV)
"Evaluation of Two Simplified Methods for Genotyping Hepatitis C Virus."
Published in Hepatitis Weekly, June 23rd, 1997
According to the authors' abstract of an article published in Journal of Medical Virology, "A number of different approaches have been used for genotyping hepatitis C virus (HCV). Two simplified methods were evaluated, both of which used polymerase chain reaction (PCR) to amplify products from the 5' non-coding region of HCV: non-isotopic restriction fragment length polymorphism (RFLP) analysis and type-specific PCR. Sixty-four viraemic patients suffering from chronic HCV infection were studied using these two techniques; 25/64 samples were further tested with a commercial serotyping ELISA based on synthetic NS4 antigen (Murex, U.K.). The results of the three typing methods were...
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Source: Hepatitis Weekly (1997-06-23)
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