Hepatitis C Diagnosis

Novel PCR method developed for detection of extrahepatic hepatitis C replication

Published in Hepatitis Weekly, March 3rd, 2003

"We established a cell culture system for the replication of hepatitis C virus (HCV) by using human T and B leukemia cell lines," researchers in the United States report.

"These 2 cell lines were infected in vitro by using HCV-positive pooled patient serum samples," said Yuan Hu and colleagues at the Food and Drug Administration Laboratory in Jamaica, New York. "HCV RNA was extracted from infected cell lines at different times after infection, and a sequence of the virus 5' untranslated region was analyzed. Hepatitis C minus-strand RNA was detected in the infected cell lines by highly strand-specific rTth (recombinant Thermus thermophilus DNA polymerase)-based...

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Source: Hepatitis Weekly (2003-03-03)

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