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Environmental Health

RNA analysis enables the detection of hepatitis A virus in oysters

Published in Hepatitis Weekly, May 5th, 2003

RNA analysis enables the detection of hepatitis A virus in oysters.

"Shellfish are readily contaminated with viruses present in water containing sewage because of the concentration effect of filter feeding. Hepatitis A virus (HAV) is the main cause of acute hepatitis worldwide and may lead to severe illness or even death. It is transmitted through fecal and oral routes and causes widespread endemic and asymptomatic infections in young children," scientists in Brazil report.

"Here we describe a method for the detection of HAV RNA in shellfish involving the extraction of total RNA from oyster meat followed by reverse transcription-polymerase chain...

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