Virology
LightCycler PCR assay offers a rapid way to quantify hepatitis B virus DNA
Published in Hepatitis Weekly, July 28th, 2003
"A highly sensitive and rapid assay has been developed to quantify hepatitis B virus (HBV) DNA, based on the fluorescence resonance energy transfer principle and real-time PCR, using the LightCycler and a pair of specific fluorescent hybridization probes. This LightCycler real-time PCR assay (LC-PCR) detected HBV DNA in a linear range from 101 to 108 copies per reaction (250-2.5 X 10(9) copies ml-1), with a rapid PCR cycling time of 35 minutes," investigators in the People's Republic of China reported.
"The assay was validated with two EUROHEP HBV DNA standards (ad and...
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Source: Hepatitis Weekly (2003-07-28)
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