Published in Hepatitis Weekly, October 20th, 2003
"Among the experimental techniques available to study the genetic variability of RNA virus populations, the most informative involve reverse transcription (RT), amplification, cloning and sequencing. The effects of several aspects of these techniques on the estimation of genetic variability in a virus population were analyzed. Hepatitis C virus populations from four patients were examined," researchers in Spain report.
"For each patient, ten series of data derived from independent PCR amplifications of a single RT reaction were obtained. The sample size of each data set was...
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