Virology
Hepatitis C virus subgenomic replicons can be grown in nonhepatic cells
Published in Hepatitis Weekly, February 16th, 2004
"Hepatitis C virus (HCV) infects liver cells and its replication in other cells is incompletely defined. Human hepatoma Huh-7 cells harboring subgenomic HCV replicons were used in somatic cell fusion experiments with human embryonic kidney 293 cells as a means of examining the permissiveness of 293 cells for HCV subgenomic RNA replication. 293 cells were generally not permissive for replication of Huh-7 cell-adapted replicons," investigators in Canada report.
"However, upon coculturing of the two cell lines, we selected rare replicon-containing cells, termed 293Rep cells, that...
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Source: Hepatitis Weekly (2004-02-16)
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