Published in Hepatitis Weekly, September 6th, 2004
According to a study from Brazil, "hepatitis A virus (HAV) replicates relatively slowly in cell culture without a cytopathic effect, a fact that limits the use of tissue culture assays. The radioimmunofocus assay is the standard method for HAV titration, although it is labor intensive and requires the use of radioisotopes. A simple, rapid and objective infectivity assay based on an in situ enzyme immunoassay (EIA) is described here for a Brazilian cell culture-adapted HAV strain (HAF-203)."
"The assay uses a peroxidase-labeled polyclonal antibody to fixed monolayers as an...
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