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Stem Cell Research

Fetal liver epithelial cells with high repopulation capacity characterized

Published in Hepatitis Weekly, September 5th, 2005

Fetal liver epithelial cells with a high repopulation capacity have been characterized.

"Epithelial cells in embryonic day (ED) 12.5 murine fetal liver were separated from hematopoietic cell populations using fluorescence-activated cell sorting (FACS) and were characterized by immunocytochemistry using a broad set of antibodies specific for epithelial cells (a-fetoprotein [ATP], albumin [ALB], pancytokeratin [PanCK], Liv2, E-cadherin, Dlk), hematopoietic/endothelial cells (Ter119, CD45, CD31), and stem/progenitor cells (c-Kit, CD34, Sca-1).

"AFP+/ALB+ cells represented approximately 2.5% of total cells and were positive for the epithelial-specific...

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