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Hepatitis C Virus

Transient-replication assay allows quantitation of hepatitis C viral RNA replication

Published in Hepatitis Weekly, December 19th, 2005

According to recently published research from Scotland, transient-replication assay allows quantitation of hepatitis C viral RNA replication.

"Dicistronic, subgenomic hepatitis C virus (HCV) replicons were constructed containing sequences from JFH1, a genotype 2a strain, that also incorporated the firefly luciferase gene under the control of the HCV internal ribosome entry site element."

"Luciferase activity in Huh-7 cell extracts containing in vitro-transcribed subgenomic JFH1 RNA was monitored over a 72 h period to examine early stages of HCV replication in the absence of any selective pressure," wrote P. Targettadams and colleagues, Medical...

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