Published in Hepatitis Weekly, June 19th, 2006
D.P. Ou and colleagues, Central South University, wrote that the study was done "to establish a sustaining hepatitis B virus X protein expressed Chang liver cell line and to explore their biological behaviours of invasive potential induced by hepatitis B virus X protein."
"Polymerase chain reaction was used to amplify the HBx gene front the whole hepatitis B virus genome. The gene was then subcloned into the eukaryotic expression vector pcDNA3.1 to construct the pcDNA3.1-HBx...
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