Published in Hepatitis Weekly, August 20th, 2007
"In this study, an infectious, selectable HCV replicon containing full-length JFH-1 cDNA was constructed. The full-genome replicon was constructed using the neomycin-resistant gene, EMCV IRES and wild-type JFH-1 cDNA. Huh7 cells were transfected with RNA synthesized in vitro, and then cultured with G418. Independent colonies were cloned to establish cell lines that replicate the full-length HCV replicon. HCV RNA replication was...
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