Published in Hepatitis Weekly, September 29th, 2008
"HBV antigen secretion was detected in the cells with transient and stable transfection by time-resolved fluoroimmunoassays (TRFIA). HBV DNA replication was examined by fluorescence quantitative PCR, and the level of HBV S mRNA was measured by semiquantitative RT-PCR. The efficiency of transient transfection of the vectors into 2.2.15 cells...
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