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Hepatitis B Virus

Reports outline hepatitis B virus research from B. Deiman and colleagues

Published in Hepatitis Weekly, October 27th, 2008

According to a study from Netherlands, "A new mechanism is described for DNA amplification using nucleic acid sequence-based amplification (NASBA (R)) including a restriction enzyme digestion and P1 primer binding directly upstream of the digestion. For hepatitis B virus (HBV), herpes simplex virus (HSV) and methicillin resistant Staphylococcus aureus (MRSA) DNA, which all show very poor amplification with normal NASBA (R), assay sensitivity was improved by a factor 100-1000 when restriction enzyme digestion was performed prior to amplification."

"For the quantitative HBV assay, in combination with the NucliSENS (R) Extractor (bioMerieux, Boxtel, The Netherlands), a 95%...

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