Hepatitis B Virus

New data from Hunan Normal University illuminate research in hepatitis B virus

Published in Hepatitis Weekly, August 10th, 2009

"Prevention of the prevalence of HB depends upon the development of efficient diagnostic reagent and preventive vaccine. Pichia pastoris offers many advantages over the other expression systems in the production of recombinant HBsAg," researchers in Changsha, People's Republic of China report.

"In this study, we reported that the recombinant P. pastoris strains were cultured in shake flasks and then scaled up in a 5.0-l bioreactor: approximately 27 mg/l of the protein and the maximal cell OD at 600 nm of 310 were achieved in the bioreactor. The recombinant HBsAg was purified by three steps of purification procedures. SDS-PAGE showed that the purified recombinant HBsAg...

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Source: Hepatitis Weekly (2009-08-10)

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