Hepatitis C Virus

New hepatitis C virus study findings recently were reported by H. Tani and co-researchers

Published in Hepatitis Weekly, May 3rd, 2010

"We have developed a continuous fluorescence assay based on fluorescence resonance energy transfer (FRET) for the monitoring of RNA helicase activity in vitro. The assay is tested using the hepatitis C virus (HCV) NS3 helicase as a model," investigators in Tsukuba, Japan report.

"We prepared a double-stranded RNA (dsRNA) substrate with a 5' fluorophore-labeled strand hybridized to a 3' quencher-labeled strand. When the dsRNA is unwound by helicase, the fluorescence of the fluorophore is emitted following the separation of the strands. Unlike in conventional gel-based assays, this new assay eliminates the complex and time-consuming steps, and can be used to simply...

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Source: Hepatitis Weekly (2010-05-03)

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