Hepatitis C Virus
Findings in Hepatitis C Virus Reported from G.L. Shi and Co-Researchers
Published in Hepatitis Weekly, September 5th, 2011
2011 SEP 5 -- According to the authors of recent research from Tokyo, Japan, "The initiation of translation in hepatitis C virus (HCV) occurs at the internal ribosome entry site (IRES) located at the 5'-end of its genomic RNA. To study the function of HCV IRES, we constructed a reporter plasmid that generates a bicistronic mRNA encoding two fluorescent proteins: cap-dependent DsRed2 and IRES-dependent Azami Green (AG)."
"We introduced the plasmid into Huh7.5.1 and HEK293 cells and measured the relative IRES activity from the ratio of AG's signal to DsRed2's in individual cells using flow cytometry. To compare our method and a conventional biochemical...
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Source: Hepatitis Weekly (2011-09-05)
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