Hepatitis B Virus

Researchers' Work from Nanyang Technological University Focuses on Hepatitis B Virus

Published in Hepatitis Weekly, October 10th, 2011

2011 OCT 10 -- "We report the development of a novel ELISA platform to quantitate hepatitis B virus X (HBx) protein refolding yields, which is critical for rational design and scaleup of aHBx bioprocess," scientists writing in the journal Analytical Biochemistry report.

"HBx refolding yields were measured by determining the amount of HBx bound to immobilized GST-p53 on a 'reduced glutathione'-functionalized maleimide surface. Refolding yields were distinguished from soluble yields, which were determined by measuring total HBx protein bound to a maleimide surface under reducing conditions," wrote A. Basu and colleagues, Nanyang Technological...

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Source: Hepatitis Weekly (2011-10-10)

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