Pseudomonas aeruginosa

Reports from W.S. Chai et al highlight recent research in Pseudomonas aeruginosa

Published in Hospital Law Weekly, August 14th, 2008

"Pseudomonas aeruginosa is a gram-negative opportunistic pathogen that is cytotoxic towards a variety of eukaryotic cells. To investigate the effect of this bacterium on monocyte, we infected human U937 cells with the P. aeruginosa strain in vitro," researchers in Shenyang, People's Republic of China report.

"To explore the expression of Bcl-2 and Bax as well as caspase-3/9 activation in the apoptosis of human U937 cells induced by P. aeruginosa, Hoechst 33258 staining and Giemsa staining as well as Flow cytometry analysis were used to determine the rate of apoptosis, and the expressions of Bcl-2 and Bax were assayed by RT-PCR and Western blotting respectively. Bax...

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Source: Hospital Law Weekly (2008-08-14)

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