Published in Immunotherapy Weekly, September 22nd, 2004
According to recent research published in the journal Biotechniques, "Here we describe plasmid vectors and selection protocols developed to allow the construction of recombinant fowlpox viruses (rFPVs) with up to three insertions of foreign DNA in the viral genome. Transient dominant selection allows the construction of recombinant viruses that do not retain the selection markers and can therefore be used for the insertion of additional genes at other sites in the viral genome."
"A SYBR Green real-time PCR sequence...
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