Chinese Academy of Sciences
Method to detect foot-and-mouth virus antibodies developed
Published in Lab Business Week, January 30th, 2005
"A codon optimized DNA sequence coding for foot-and-mouth disease virus (FMDV) capsid protein complex epitopes of VP1 amino acid residues 21-40, 135-160, and 200-213 was genetically fused to the C-terminal end of a glutathione-S-transferase (GST) gene in pGEX-6P-1 vector with the synonymous codons preferred by Escherichia coli. The gene was synthesized using PCR and subsequently expressed in E. coli producing an intracellular, soluble fusion protein...
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Source: Lab Business Week (2005-01-30)
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