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Cloning

Studies from Hayashibara Biochemical Laboratories, Inc. further understanding of cloning

Published in Lab Business Week, January 21st, 2007

A report, "Cloning, sequencing, and expression of the genes encoding an isocyclomaltooligosaccharide glucanotransferase and an alpha-amylase from a Bacillus circulans strain," is newly published data in Bioscience, Biotechnology, and Biochemistry. "The gene for a novel glucanotransferase, isocyclomaltooligosaccharide glucanotransferase (IgtY), involved in the synthesis of a cyclomaltopentaose cyclized by an alpha-1,6-linkage [ICG5; cyclo-{-- >6)-alpha-D-Glcp-(1-- >4)-alpha-D-Glcp-(1-- >4)-alpha-D-Glcp-(1-- >4)-alpha-D-Glcp-(1-- >4)-alpha-D-Glcp-(1-- >}] from starch, was cloned from the genome of B. circulans AM7. The IgtY gene, designated igtY, consisted of 2,985 bp...

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