Shandong Eye Institute & Hospital, Qingdao
Antisense constructs abate excess lens epithelial cell proliferation
Published in Lab Law Weekly, August 19th, 2005
Researchers in the People's Republic of China conducted a study "to investigate the effects of adenovirus-mediated transfer of antisense c-myc construct on human lens epithelial B-3 (HLE B-3) cell proliferation, apoptosis and cell cycle. HLE B-3 cell cultures were transduced with replication-defective adenovirus bearing either a nuclear-targeted beta-galactosidase (Ad-lacZ) or an antisense c-myc construct (Ad-AS-myc)."
"The presence of beta-galactosidase activity in the transduced cultures was detected by immunohistochemical X-Gal staining, while c-myc mRNA and protein expression...
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Source: Lab Law Weekly (2005-08-19)
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