Published in Malaria Weekly, November 28th, 2005
"We have developed a rapid and simple method for determining accurately the proportions of alleles or individual malaria clones in a mixed infection," scientists writing in the journal Molecular and Biochemical Parasitology report.
"The technique uses a nested PCR reaction to amplify, from parasite mixtures, alleles of genes differing by single nucleotide polymorphisms, simultaneously, using common primers to non-polymorphic sequences. The mixed products are sequenced, and the heights of fluorescence peaks associated with...
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