Life Sciences

Research on life sciences detailed by S.E. Shokoples and co-authors

Published in Malaria Weekly, May 18th, 2009

According to recent research from Edmonton, Canada, "The implementation of real-time PCR for the diagnosis of malaria has been hampered by poor sensitivity for the detection of mixed infections. We have optimized a method that enhances the sensitivity of detection of minor species in mixed infections within a single multiplex reaction."

"Our assay uses species-specific forward primers in combination with a conserved reverse primer and largely overcomes primer competition for the minor species DNA. With a blind panel of clinical samples, we successfully identified the species in 13/16 mixed infections. This assay was further validated with 91 blood samples and...

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Source: Malaria Weekly (2009-05-18)

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