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Biochemistry

Research on biochemistry reported by scientists at GenoFunction, Inc.

Published in Medical Device Business Week, February 14th, 2007

Research findings, "Uniform amplification of multiple DNAs by emulsion PCR," are discussed in a new report. According to a study from Tsukuba, Japan, "When several DNAs are amplified by PCR in one PCR tube, biased amplification is known to occur because amplification efficiency differs from one DNA to another. Therefore, we conducted PCR in the water in oil-emulsion (W/O emulsion) to examine whether the procedure allows the uniform amplification of several DNAs."

"In the amplification of a model library consisting of two clones, the emulsification of the PCR mixture successfully reduced the difference in its amplification efficiency to approximately one-seventh the...

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