Published in Medical Device Business Week, September 9th, 2009
"The bee venom was separated by linear gradient 5-80% B at 30 min (eluent A-0.1% TFA in water, eluent B-0.1% TFA in acetonitrile:water (80:20)). The flow rate of mobile phase was maintained at 1 mL min(-1), injection volume: 40 mu L, separation temperature: 25 degrees C. The analysis was monitored at 220 nm. Several honeybee venom constituents were separated and the content of four of them (apamine, mast cell degranulating peptide, phospholipase...
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