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Bioassay detects soluble MAdCAM-1 in body fluids

Published in Medical Devices and Surgical Technology Week, November 7th, 2004

Researchers have developed a bioassay to detect soluble MAdCAM-1 in body fluids.

According to recent research from New Zealand, "Mucosal addressin cell adhesion molecule (MAdCAM-1) is a key player in mediating the infiltration of leucocytes into chronically inflamed tissues. Five anti-MAdCAM-1 monoclonal antibodies (mAb), designated 17175, 201F7, 314G8, 377D10, and 355G8, were generated by fusion of P3 x 63Ag8.653 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human MAdCAM-1-Fc. The latter four mAb recognize the ligand-binding first Ig domain, and block T -cell adhesion to MAdCAM-1."

"The non-blocking mAb 17F5 recognizes...

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