Enzymology
Novel immunoassay facilitates cytochrome P450 quantification
Published in Medical Devices and Surgical Technology Week, November 28th, 2004
"Cytochrome P450-dependent monooxygenases (P450s) are integral membrane proteins typically expressed at low levels both in vivo and by heterologous expression systems, often making quantification of these enzymes challenging," scientists in Indiana explained. "Since the time of their discovery, P450s have typically been quantified by their carbon monoxide (CO) difference spectra."
"Although this technique is reliable, it requires quantities of enzyme that are sometimes difficult to obtain, and spectroscopic instruments and expertise frequently unavailable in laboratories whose...
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