Physiology
Splicing of U12-type introns analyzed, influence on mRNA decay revealed
Published in Medical Devices and Surgical Technology Week, March 20th, 2005
"Metazoan cells have two pathways for intron removal involving the U2- and U12-type spliceosomes, which contain mostly nonoverlapping sets of small nuclear ribonucleoproteins.
"We show that in vitro splicing of a U12-type intron assembles an exon junction complex (EJC) that is comparably positioned and contains many of the same components as that deposited by the U2-type spliceosome. The presence of a U12-type intron downstream of a premature termination codon within an open reading frame (ORF)...
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