Blood-Brain Barrier
Puromycin-based purification improves in vitro models
Published in Medical Devices and Surgical Technology Week, May 29th, 2005
"One of the main difficulties" with primary RBEC cultures "is obtaining pure cultures," neuroscientists in France explained. "The variation in purity limits the achievement of in vitro models of the rat blood-brain barrier."
"As P-glycoprotein expression is known to be much higher in RBECs than in any contaminating cells," N. Perriere and colleagues at Rene Descartes University "tested the effect of five P-glycoprotein substrates (vincristine, vinblastine, colchicine, puromycin and doxorubicin) on RBEC cultures, assuming that...
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