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Chromatography

Findings from N.A. Devries and co-authors broaden understanding of chromatography

Published in Medical Devices and Surgical Technology Week, March 9th, 2008

"A sensitive and selective reversed-phase high-performance liquid chromatographic (HPLC) assay has been developed and validated for quantification of total topotecan in human and mouse plasma and in mouse tissue samples. Isocratic separation was achieved on a Zorbax SB-C-18 column and topotecan was monitored fluorimetrically," researchers in Netherlands report.

"Two ranges of calibrations curves were used to determine lower levels of topotecan more accurately. Acceptable accuracy and precision was achieved for all matrices. Topotecan was stable upon repeated freeze-thawing for three cycles or storage for 24 h at ambient temperatures in spiked plasma samples and tissue...

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