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Enzyme Research

Research from Srinakharinwirot University, Medical Department has provided new information about enzyme research

Published in Medical Imaging Law Weekly, April 14th, 2009

"A thermophilic pectate lyase, Pel SWU, was isolated from a culture filtrate of Bacillus sp. RN1 isolated from a hot spring in Ranong Province, Thailand," scientists in Bangkok, Thailand report.

"The enzyme was purified to homogeneity using cation-exchange and hydrophobic column chromatographies. The molecular mass of Pel SWU was estimated to be 33 kDa. The specific substrate was demethylated galacturonic acid. The enzyme was stable at pH 4.0-10.0 and at temperatures up to 70 degrees C in the presence of calcium and polygalacturonic acid (PGA). The optimum pH and temperature were 10.0 and 90 degrees C. The pel gene encoding Pel SWU was 1,023 lip, which corresponds to...

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