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Spectroscopy

New research on spectroscopy from China University summarized

Published in Medical Imaging Week, January 5th, 2008

"Glutamine-free culture of Vero cells has previously been shown to cause higher cell yield and lower ammonia accumulation than that in glutamine-containing culture. Nitrogen metabolism of asparagine and glutamate as glutamine replacer was studied here using nuclear magnetic resonance (NMR) spectroscopy," scientists in Shanghai, People's Republic of China report.

"N-15-labelled glutamate or asparagine was added and their incorporation into nitrogenous metabolites was monitored by heteronuclear multiple bond coherence (HMBC) NMR spectroscopy. In cells incubated with L-[N-15] glutamate, the N-15 label was subsequently found in a number of metabolites including alanine,...

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