"Development and Application of a Reverse Transcriptase-PCR Assay for Laboratory Diagnosis of Colorado Tick Fever (CTF) Virus in Acute Clinical Specimens."

According to an abstract submitted by the authors to the 13th Annual Clinical Virology Symposium and Annual Meeting of the Pan American Society for Clinical Virology, held April 27-30, 1997, in Clearwater Beach, Florida, "Objective: To develop and apply RT-PCR for diagnosis of CTF in acute clinical specimens. Methods: RNA was extracted from multiple CTF virus isolates and related viruses by using a QIAamp viral RNA extraction kit. These were subjected to a RT-PCR method developed for use with ds-RNA, and amplicons were detected by using ethidium bromide-containing 1.5% agarose gels. The technique was applied to the detection of viral RNA extracted from human sera, and...

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